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Allender, Matthew C. Kane, Lauren P 2016-07-07T19:53:05Z 2016-07-07T19:53:05Z 2016-03-18 2016-05 Herpesviruses are ubiquitous, well described pathogens in captive chelonians worldwide, but their importance on free-ranging populations are less defined. In this thesis, a quantitative PCR was developed that detected a 58 base pair segment of the DNA polymerase gene segment of Terrapene herpesvirus 1 (Order: Herpesvirales; Family: Herpesviridae; Subfamily: Alphaherpesvirinae; Genus: Scutavirus). This assay was used to estimate prevalence of herpesvirus infection in 409 free-ranging eastern box turtles (Terrapene carolina carolina) from Tennessee and Illinois. The overall prevalence in this study population was 31.3% (95% confidence interval: 27-36%), with a significantly higher prevalence in July (52.3%; 95% CI: 41-59%) compared to May (13.3%; 95% CI: 5-15%) and September (34.4%; 95% CI: 29-47%). Clinical signs recorded in box turtles were not significantly associated with herpesvirus infection and may be attributed to a latency period. The work presented in this thesis aids in characterizing the epidemiology of herpesvirus in chelonians. Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-07-07 without embargo terms The student, Lauren Kane, accepted the attached license on 2016-03-16 at 11:07. The student, Lauren Kane, submitted this Thesis for approval on 2016-03-16 at 12:02. This Thesis was approved for publication on 2016-03-18 at 13:11. DSpace SAF Submission Ingestion Package generated from Vireo submission #9106 on 2016-07-07 at 13:28:16 Made available in DSpace on 2016-07-07T19:53:05Z (GMT). No. of bitstreams: 2 KANE-THESIS-2016.pdf: 11426690 bytes, checksum: 2f84242a145943eac2e9685ad4f7de90 (MD5) LICENSE.txt: 4208 bytes, checksum: 2c4ae08d6f53f8b190e8bbf65dfff302 (MD5) Previous issue date: 2016-03-18 application/pdf http://hdl.handle.net/2142/90487 en Copyright 2016 LAUREN P. KANE Epidemiology herpesvirus Eastern box turtle qPCR Terrapene carolina disease Investigating the epidemiology of Terrapene herpesvirus 1 in freeranging eastern box turtle populations text text Comparative Biosciences VMS - Comparative Biosciences University of Illinois at Urbana-Champaign Thesis M.S.