2026-09-22T17:03:37Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/90866 2023-07-11 col_2142_5131 com_2142_5130

text text Rodriguez-Zas, Sandra Rodriguez-Zas, Sandra Wheeler, Matthew Villamil, Maria Steelman, Andrew Caetano, Kelsey 2016-07-07T21:04:23Z 2016-07-07T21:04:23Z 2018-07-08T09:15:33Z 2016-04-20 2016-05 Addiction to activity and to psychoactive substances share psychologically and physiological characteristics. A transcriptome study compared a mouse line selected for high voluntary activity to a control line in environments restricting or enabling a rewarding activity. Results offered insights into genetic factors behind activity dependence and provided a model for understanding addiction and other reward-dependent behaviors.Most genes differentially expressed betweenactivity genotypes were only moderately differentially expressed between activity environments, suggesting that environmental effects were not confounded with activity genotype effects. Adora2a had a significant genotype-by-environment interaction effect evidenced by over-expression in the activity genotype relative to control in high activity environment and under-expression in the low activity environment.Our findings of differentially expressed genes related to dopaminergic transmission between activity genotypes support the association between these genes and activity. Acentral theme from the functional analysis of activity genotype-environment was neuron morphogenesis. Gene network analysis identified connected genes that exhibited similar (e.g. Lhx9) and opposite (e.g. Nrgn) expression patterns between activity genotypes across reward availability environment.Our findings suggest that some transcriptomic changes in mice selected for high voluntary activity are shared with other reward-dependent behaviors. Results from this study support that high voluntary activity selection lines in mice are a helpful model to understand molecular mechanisms behind addition. Also, identification of genes and biological processes associated with both high voluntary activity and the pleasurable neurological response to physical activity may allow forthe development of drugs which make it more pleasurable for people to exercise or less pleasurable to be sedentary as a treatment approach for overweight/obesity as well as a way to improve health overall in the general population. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-05-01 The student, Kelsey Caetano, accepted the attached license on 2016-02-04 at 00:37. The student, Kelsey Caetano, submitted this Dissertation for approval on 2016-02-04 at 01:33. This Dissertation was approved for publication on 2016-04-20 at 10:29. DSpace SAF Submission Ingestion Package generated from Vireo submission #9066 on 2016-07-07 at 14:16:02 Made available in DSpace on 2016-07-07T21:04:23Z (GMT). No. of bitstreams: 3 CAETANO-DISSERTATION-2016.pdf: 11077150 bytes, checksum: 78c887b356d05e75284a626adcc612e9 (MD5) LICENSE.txt: 4211 bytes, checksum: 7962894354d7cb0c707c3906da14d34d (MD5) PROQUEST_LICENSE.txt: 4557 bytes, checksum: f0fc6346ade3e40aa0116cffccd64ee2 (MD5) Previous issue date: 2016-04-20 Embargo set by: Seth Robbins for item 93219 Lift date: 2018-07-07T21:04:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 93219 Lift date: 2018-07-07T21:14:52Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 93219 Lift date: 2018-07-07T21:18:16Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 93219 on 2018-07-08T09:15:33Z. application/pdf http://hdl.handle.net/2142/90866 en Copyright 2016 Kelsey Caetano Bioinformatics Computational Biology Genetics Next-Generation Sequencing RNA-Sequencing Applications of next-generation sequencing technologies and advanced analytical methods to understand gene by environment interactions Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.