2026-09-21T13:45:57Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/92665 2023-07-11 col_2142_5131 com_2142_5130

Zhang, Yuanhui Schideman, Lance Charles Martins, Marcio Arêdes Guan, Shaochen 2016-11-10T17:49:11Z 2016-11-10T17:49:11Z 2016-07-21 2016-08 Microalgae is a promising biofuel source with high photosynthetic efficiency, and it often contains high-value substances for nutritional, pharmaceutical and medical applications. In order to improve the economic viability of the spirulina utilization, this study investigated a cost competitive biotechnological process for C-phycocyanin (C-PC) extraction from spirulina platensis. The residual biomass after C-PC extraction was converted into bio-crude oil via hydrothermal liquefaction (HTL). The HTL bio-crude oil obtained from the original spirulina feedstock and the residual after the C-PC extraction were compared. A rapid and efficient process for extraction and purification of food-level C-PC from spirulina platensis was developed in this study. The process conditions include: 1) freeze the spirulina 3 hours to extract the crude protein; 2) add 80 g/L (w/v) activated carbon to the crude extract; 3) use a vacuum filter with a 0.22 μm pore size membrane to collect the extract; and 4) freeze dry the extract to get the C-PC powder. The yield of the C-PC production is 27% and the cost (exclude labor) for entire process is $26.1/kg. After C-PC extraction, there is still 63% dry biomass left which was used for conversion into bio-crude oil via HTL. The HTL bio-oil products distribution as well as their composition, reaction pathways and energy recovery via HTL were investigated. HTL was conducted at temperatures range from 260˚C to 300˚C at 0.7 MPa N2 initial pressures. The highest bio-crude oil yield of 38 % (based on dry volatile matter) was obtained at 300˚C. The highest higher heating value is 37.1 MJ/kg occurred at 300°C reaction temperature. Elemental analysis revealed that the decarboxylation and denitrification may be dominant from 260˚C to 300°C following repolymerization governing at higher temperature; TG analysis showed that approximately 75.4 % distilled bio-crude products were in the range of 200-550°C. These distillates can be further upgraded for transportation fuels. Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-11-09 without embargo terms The student, Shaochen Guan, accepted the attached license on 2016-07-20 at 11:49. The student, Shaochen Guan, submitted this Thesis for approval on 2016-07-20 at 12:01. This Thesis was approved for publication on 2016-07-21 at 09:03. DSpace SAF Submission Ingestion Package generated from Vireo submission #10036 on 2016-11-09 at 10:25:56 Made available in DSpace on 2016-11-10T17:49:11Z (GMT). No. of bitstreams: 2 GUAN-THESIS-2016.pdf: 3090110 bytes, checksum: 6f36d77e92c3136ec841b4d7df130bae (MD5) LICENSE.txt: 4210 bytes, checksum: 97773293cc5fccbaa2ea196cb11bc13d (MD5) Previous issue date: 2016-07-21 application/pdf http://hdl.handle.net/2142/92665 en Copyright 2016 Shaochen Guan Extraction Purification Spirulina Phycocyanin Bio-crude oil Hydrothermal liquefaction Extracting phycocyanin from spirulina and hydrothermal liquefaction of its residues to produce bio-crude oil text text Engineering Administration Agricultural & Biological Engr University of Illinois at Urbana-Champaign Thesis M.S.