2026-09-20T17:09:58Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/92878 2023-07-11 col_2142_5131 com_2142_5130

Kong, Hyunjoon Leckband, Deborah E. Qin, Ellen C. 2016-11-10T18:27:11Z 2016-11-10T18:27:11Z 2018-11-11T10:15:19Z 2016-07-22 2016-08 In the case of injury, aging, or disease, tissues in the body may have a limited capability to regenerate. As a result, great interest have been put into tissue engineering as a way to assemble functional constructs that restore, maintain, or improve those tissues. Certain success was made to modulate neural differentiation of stem cells using soluble factors and cell adhesion matrix properties. These differentiation studies are often dependent on the density of cells plated on the substrate, which implies that there is an important role of cadherin-modulated cell-cell adhesion in regulating stem cell differentiation levels. As a result, the goal for this research is to develop a hydrogel platform which integrates the effects of N-cadherin and matrix stiffness to modulate cell differentiation. To create this system, Fc-tagged N-cadherins are attached to alginate gels of varying stiffness through Protein A, which is chemically bound to the surface. Different cells known to express N-cadherin were seeded onto the gels, with the bone marrow stromal cells (BMSCs) showing the best adhesion properties. This study thus demonstrates that N-cadherin substrates can be used to promote cell adhesion, and may be useful for differentiation studies with BMSCs. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2018-08-01 The student, Ellen Qin, accepted the attached license on 2016-07-22 at 09:53. The student, Ellen Qin, submitted this Thesis for approval on 2016-07-22 at 09:55. This Thesis was approved for publication on 2016-07-22 at 13:36. DSpace SAF Submission Ingestion Package generated from Vireo submission #10059 on 2016-11-10 at 12:21:06 Made available in DSpace on 2016-11-10T18:27:11Z (GMT). No. of bitstreams: 2 QIN-THESIS-2016.pdf: 1200506 bytes, checksum: 10e3b8593c69fbf8ef2684bbd55c43d5 (MD5) LICENSE.txt: 4206 bytes, checksum: e32c4392b9a751c8457d44b0de2b8cb0 (MD5) Previous issue date: 2016-07-22 Embargo set by: Seth Robbins for item 95298 Lift date: 2018-11-10T18:28:02Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 95298 on 2018-11-11T10:15:19Z. application/pdf http://hdl.handle.net/2142/92878 en Copyright 2016 Ellen Qin N-cadherin Hydrogel Stiffness Bone marrow stromal cell Engineered N-cadherin substrates for stem cell differentiation text text M.S. Thesis Chemical & Biomolecular Engr Chemical Engineering University of Illinois at Urbana-Champaign