2026-09-22T02:06:20Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/97431 2023-07-11 col_2142_5131 col_2142_14828 com_2142_5130 com_2142_14827 com_2142_14793 com_2142_8903

Corfts, Antony R Corfts, Antony R Gennis, Robert Gruebele, Martin Pogorelov, Taras Rose, Stuart Wallace 2017-08-10T19:15:41Z 2017-08-10T19:15:41Z 2017-04-21 2017-05 This work seeks to duplicate a realistic membrane for a more natural model of the Rb. sphaeroides bc1 complex which in past studies has lacked several details in composition of the fatty acids and relative quantities of each lipid. Past studies have shown some distortion on MD relaxation relating to a large void volume in the protein structures. In this model we have set up the membrane with the complement of lipids reported for the chromatophore membrane, and have taken steps to ameliorate the structural distortions on relaxation of the protein by populating the void with a complement of lipids. The MD model is used to determine diffusion constants and motions of the system in preparation for calculating potentials of mean force for wild type and ISP tether mutants. The current kinetic model provides a kinetic and thermodynamic understanding of the rate-limiting reaction, and associated partial processes that lead to successive turnovers. Since both bacterial and mitochondrial complexes have essentially the same catalytic core, their mechanisms are essentially similar, and a better understanding of the bacterial system can be extrapolated to the context of mitochondrial function, and medically important roles in cellular physiology, cardiovascular disease, apoptosis, and diseases associated with aging. Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2017-08-10 without embargo terms The student, Stuart Rose, accepted the attached license on 2017-04-21 at 10:35. The student, Stuart Rose, submitted this Dissertation for approval on 2017-04-21 at 10:49. This Dissertation was approved for publication on 2017-04-21 at 13:56. DSpace SAF Submission Ingestion Package generated from Vireo submission #10965 on 2017-08-10 at 13:43:39 Made available in DSpace on 2017-08-10T19:15:41Z (GMT). No. of bitstreams: 3 ROSE-DISSERTATION-2017.pdf: 4772321 bytes, checksum: a815d5a4097616fddd4ea66d9ea58892 (MD5) LICENSE.txt: 4208 bytes, checksum: 717529b57e2bb0b3dc38b90679dfab95 (MD5) PROQUEST_LICENSE.txt: 4554 bytes, checksum: 403fac01e4ec2b6d9a04f4eaf922214e (MD5) Previous issue date: 2017-04-21 application/pdf http://hdl.handle.net/2142/97431 en Copyright 2017 Stuart Rose Bc1 complex Mechanism Kinetics Membrane Biochemically realistic MD and kinetic models of the Rhodobacter sphaeroides bc1 complex text text School of Molecular & Cell Bio Biophysics & Computnl Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.