2026-09-23T08:07:50Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/97561 2023-07-11 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

Brieher, William M. Brieher, William M. Freeman, Brian C. Gillette, Martha U. Saif, Taher A. Kemp, James Peter 2017-08-10T19:51:51Z 2017-08-10T19:51:51Z 2019-08-11T09:15:09Z 2017-04-14 2017-05 Cells use actin to provide structure and stability to the membrane, to drive motility, and to adhere to, pull on, and reorganize the extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for contractile actin arrays, actually inhibits bundling of actin filaments into stress fibers in epithelial cells. I show that α-actinin-4 accomplishes this by inhibiting tropomyosin dependent stabilization of filamentous actin, and maintains a dynamic basal actin array through promoting actin disassembly. Lastly I demonstrate that α-actinin-4 localization in cells can be modulated by phosphorylation of its actin-binding domain. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01 The student, James Kemp, accepted the attached license on 2017-04-12 at 10:56. The student, James Kemp, submitted this Dissertation for approval on 2017-04-12 at 11:01. This Dissertation was approved for publication on 2017-04-14 at 10:34. DSpace SAF Submission Ingestion Package generated from Vireo submission #10717 on 2017-08-10 at 14:30:25 Made available in DSpace on 2017-08-10T19:51:51Z (GMT). No. of bitstreams: 3 KEMP-DISSERTATION-2017.pdf: 3928703 bytes, checksum: 42ff6b1aa25b39a80223800bbc129ad5 (MD5) LICENSE.txt: 4207 bytes, checksum: cdc3599210342404f5b5a2758d44dd9f (MD5) PROQUEST_LICENSE.txt: 4553 bytes, checksum: eeda0d2631c3e513192793a7121b3115 (MD5) Previous issue date: 2017-04-14 Embargo set by: Colleen Fallaw for item 102614 Lift date: 2019-08-10T21:25:30Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 102614 on 2019-08-11T09:15:09Z. application/pdf http://hdl.handle.net/2142/97561 en Copyright 2017 James Peter Kemp Jr Stress fibers Alpha-actinin A study on the modulation of alpha-actinin and filamentous actin text text Cell & Developmental Biology Cell and Developmental Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.