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University of Illinois Urbana-Champaign

Liver Organoid Assembly for Toxicity Assessment of Silver Nanoparticles

Bidon, Miya K.; Sullivan, Kathryn M.; Park, Jooyen; Kong, Hyunjoon

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https://hdl.handle.net/2142/98074

Description

Title
Liver Organoid Assembly for Toxicity Assessment of Silver Nanoparticles
Author(s)
  • Bidon, Miya K.
  • Sullivan, Kathryn M.
  • Park, Jooyen
  • Kong, Hyunjoon
Issue Date
2017
Keyword(s)
  • Silver nanoparticles
  • 3D culture
  • HepG2
  • extracellular matrices
  • mescenchymal stem cells
Date of Ingest
2017-09-27T15:19:05Z
Abstract
Due to their interaction with water, nanoparticles experience a compounding cycle of being absorbed in multiple organisms, most notably commercial fish and humans. The purpose of this research is to assemble clusters of hepatic cells to enable the assessment of the toxicity levels of silver nanoparticles (AgNPs). For this analysis, we cultured liver cancer cells (HepG2) into decellularized MSC matrices onto non-adherent surfaces to maintain cellular phenotype, increase cell proliferation, and promote high differentiation potential. Based on this research, spheroid MSC-DCM has shown to provide more physiologically relevant conditions to enhance the growth and detoxification activity of liver cancer cells. From this, we were able to quantitatively and qualitatively analyze the increased toxicity levels of AgNPs between size 40 and 130 nm.
Type of Resource
image
Genre of Resource
Conference Poster
Permalink
http://hdl.handle.net/2142/98074
Copyright and License Information
Copyright 2017 Miya K. Bidon

Owning Collections

Undergraduate Research Symposium 2017 PRIMARY