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Modeling heterogeneity in the microbial world
Cole, Jr., John Andrew
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https://hdl.handle.net/2142/99301
Description
- Title
- Modeling heterogeneity in the microbial world
- Author(s)
- Cole, Jr., John Andrew
- Issue Date
- 2017-10-10
- Director of Research (if dissertation) or Advisor (if thesis)
- Luthey-Schulten, Zaida
- Doctoral Committee Chair(s)
- Goldenfeld, Nigel
- Committee Member(s)
- Chemla, Yann
- Kuehn, Seppe
- Department of Study
- Physics
- Discipline
- Physics
- Degree Granting Institution
- University of Illinois at Urbana-Champaign
- Degree Name
- Ph.D.
- Degree Level
- Dissertation
- Keyword(s)
- Stochastic gene expression, metabolism
- Abstract
- No two living cells are identical. Like tiny, squishy snowflakes, even seemingly identical cells (belonging to isogenic populations, and living under macroscopically similar conditions), will experience variations in their local microenvironments and in the numbers of gene copies, messenger RNAs, proteins, and other macromolecules that constitute their make up, and importantly, dictate their behavior. In this thesis I will employ the methods of systems biology, stochastic simulation, and mathematical analysis to investigate some of the causes and outcomes of this type of biological variability in the microbial world. This document is divided into four main chapters. The first focuses on how the stochastic expression of metabolic enzymes affects the growth and metabolic pathway usage of individual Escherichia coli cells. The second chapter deals with how E. coli cells in colonies diverge behaviorally as a function of their location, and how they naturally tend to cooperate in a previously unknown form of crossfeeding. Finally, the third and fourth chapters deal with details of how gene expression stochasticity itself arises, with an eye toward the effects that DNA replication have on mRNA and protein statistics (and what that means for interpreting single molecule experiments).
- Graduation Semester
- 2017-12
- Type of Resource
- text
- Permalink
- http://hdl.handle.net/2142/99301
- Copyright and License Information
- Copyright 2017 John A. Cole Jr.
Owning Collections
Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at IllinoisDissertations and Theses - Physics
Dissertations in PhysicsManage Files
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