Development of a Long-Term Anesthesia Protocol for Swine

Ruston-Bray, Alyssa L. A.; Thomas, Faith; Hayes, Courtney; Schook, Lawrence; Dailey, Megan

Permalink

https://hdl.handle.net/2142/99708 Copy

Description

Title

Development of a Long-Term Anesthesia Protocol for Swine

Author(s)

• Ruston-Bray, Alyssa L. A.
• Thomas, Faith
• Hayes, Courtney
• Schook, Lawrence
• Dailey, Megan

Issue Date

2018-04

Keyword(s)

• Anesthesia
• swine

Abstract

The use of swine as a preclinical model is becoming standard practice because of the similarity to humans in size, anatomy, physiology, and genetics. Anesthesia is frequently required for swine in research due to the nature of the invasive procedures that are performed. Developing a protocol which considers the physiological effects of the pharmacological agents and the proper way to administer these agents is important when designing experiments. We aimed to develop an anesthesia protocol for magnetic resonance imaging (MRI) and magnetic resonance elastography (MRE) of swine that allows the animals to be anesthetized for multiple hours and for the administration of pharmacological and contrast agents to be done while the animal is in the MRI scanner. We used three pigs weighing 68, 40, and 30 kg. We found that an initial intramuscular injection of telazol/ketamine/xylazine (TKX) at a dose of 1ml/23kg, followed by endotracheal intubation and 1.5% isoflurane+1.5ml/min oxygen successfully anesthetized all swine for up to 6hrs. For administering contrast agents during an MRI, an auricular vein catheter was found to be effective but a jugular cutdown is possible when requiring the injection of larger volumes. Physiological monitoring using a pulse oximeter and rectal thermometer every 10-15 minutes proved effective. We also found that the animals could not tolerate TKX post-isoflurane anesthesia, which is something to consider if isoflurane anesthesia cannot be continued during transport. Development of this protocol allows for the ease of the anesthetizing process, an understanding of the physiological factors and the effects of anesthesia.

Type of Resource

• text
• image

Language

en

Permalink

http://hdl.handle.net/2142/99708

Owning Collections