University of Illinois Urbana-Champaign

Discovery of Glycocin from Pathway Refactoring

Ho, Sherri; Ren, Hengqian

Content Files
Ho2018Symposium.pdf

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https://hdl.handle.net/2142/99957

Description

Title
Discovery of Glycocin from Pathway Refactoring
Author(s)
  • Ho, Sherri
  • Ren, Hengqian
Contributor(s)
Zhao, Huimin
Issue Date
2018-04
Keyword(s)
  • Molecular and Cellular Biology
  • Synthetic Biology
  • Science
  • Natural Product
  • Glycocin
  • Protein Purification
Date of Ingest
2018-05-21T12:42:27Z
Abstract
Natural products have been playing an indispensable role in pharmaceuticals and human therapeutics for decades. However, because of the continuous development of drug resistance, high throughput discovery of natural products is always an urgent but seemingly impossible challenge. Synthetic biology, with its power to engineer biosynthetic pathways, has proven to be a promising solution to this issue. One type of natural product that will be investigated are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Glycocins, a class of RiPPs, are a form of bacterial toxins that can inhibit the growth of many bacterial strains. In this study, we used heterologous expression to engineer the pathway of glycocin from Geobacillus followed by fermentation, fractionation, and purification of the glycocin. In the end, we discovered a glycocin from the thermophilic bacteria Geobacillus which has never been done before.
Type of Resource
image
Genre of Resource
Conference Poster
Permalink
http://hdl.handle.net/2142/99957
Sponsor(s)/Grant Number(s)
MCB SROP
Copyright and License Information
  • Copyright 2018 Sherri Ho
  • Copyright 2018 Hengqian Ren

Owning Collections

Undergraduate Research Symposium 2018 PRIMARY