Shedding light on E. coli phototaxis

Perlova, Tatyana Yurievna

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https://hdl.handle.net/2142/100891 Copy

Description

Title

Shedding light on E. coli phototaxis

Author(s)

Perlova, Tatyana Yurievna

Issue Date

2018-01-22

Director of Research (if dissertation) or Advisor (if thesis)

• Gruebele, Martin
• Chemla, Yann R.

Doctoral Committee Chair(s)

Rao, Christopher

Committee Member(s)

Shukla, Diwakar

Department of Study

Physics

Discipline

Physics

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Escherichia coli, chemotaxis, phototaxis

Abstract

My thesis work has been focused on phototaxis response in E. coli. I will review the current state of knowledge on chemotaxis and phototaxis and discuss the motivation behind this project in Chapter 1. In Chapters 2 and 3, I will describe a high-throughput framework for studying phototaxis in bacteria, which consists of the experimental setup and automated routine for data analysis, that I have developed over the course of this project. In Chapter 4, I will present experimental results that allowed us to pinpoint the role of individual components of the chemotactic network in phototaxis response and to characterize power dependence of the response. In Chapter 5, I will discuss the role of receptor-receptor interaction in determining the integrated phototactic response. In Chapter 6, I will quantify the effect of light on E. coli swimming velocity and its potential implication. In Chapter 7, I will discuss how various external parameters affect E. coli phototaxis response. Finally, in Chapter 8, I will present some preliminary results and discuss potential future directions of this project.

Graduation Semester

2018-05

Type of Resource

text

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http://hdl.handle.net/2142/100891

Copyright and License Information

Copyright 2017 Tatyana Perlova

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