Whole genomic structural variant calling in soybean: Analysis on 481 different soybean lines

Istanto, Dave Deandre

Permalink

https://hdl.handle.net/2142/107902 Copy

Description

Title

Whole genomic structural variant calling in soybean: Analysis on 481 different soybean lines

Author(s)

Istanto, Dave Deandre

Issue Date

2020-04-23

Director of Research (if dissertation) or Advisor (if thesis)

Hudson, Matthew

Committee Member(s)

• Mainzer, Liudmila
• Clough, Steven

Department of Study

Crop Sciences

Discipline

Bioinformatics

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• Soybean
• Transposon
• Structural variation
• Variant calling

Abstract

Soybean (Glycine max) is an important crop worldwide, with utilities as protein source and rotation crop. Often, soybean variant discovery is conducted using alignment methods and largely yields short variants such as SNP(s) and short indel(s). We conducted variant discovery on 481 soybean lines using both alignment and assembly methods. We used the Sentieon Haplotyper algorithm for our alignment-based variant calling and Cortex-var for our assembly-based variant discovery and found many more short variants compared to structural variants: medians 2,728,393 and 3,972 variants respectively. Additionally, we provide the user-friendly workflow script together with full-documentation for Cortex-var assembly-based variant discovery. Additionally, post variant discovery analysis was conducted with a focus on transposable element activities in the same 481 soybean lines. Structural variants were filtered for transposable element, and then the transposable elements identified were compared between elite soybean lines and Glycine soja lines, and then the transposable element activity of G. soja lines was statistically higher compared to the soybean elite lines. Consequently, it is possible to distinguish the difference between elite soybean lines, landraces, and G. soja lines through structural variant information. It was also found that some transposable elements that might be potentially disrupting genes with functions such as carbohydrate metabolism and embryo development with high activities in the leaves, pods, and pod shells.

Graduation Semester

2020-05

Type of Resource

Thesis

Permalink

http://hdl.handle.net/2142/107902

Copyright and License Information

Copyright 2020 Dave Istanto

Owning Collections