University of Illinois Urbana-Champaign

Development of chemical tools for studying biology with light: Photoactivatable donors and photoacoustic probes

Smaga, Lukas P.

Content Files
SMAGA-DISSERTATION-2020.pdf

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https://hdl.handle.net/2142/108239

Description

Title
Development of chemical tools for studying biology with light: Photoactivatable donors and photoacoustic probes
Author(s)
Smaga, Lukas P.
Issue Date
2020-04-09
Director of Research (if dissertation) or Advisor (if thesis)
Chan, Jefferson
Doctoral Committee Chair(s)
Chan, Jefferson
Committee Member(s)
  • Mitchell, Douglas A
  • Sarlah, David
  • Zimmerman, Steven C
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2020-08-27T00:49:52Z
Keyword(s)
Formaldehyde, Photoacoustic Imaging
Abstract
Chemical tools are essential for the study of complex biological processes. Generally, this is achieved through the controlled perturbation of a biological system and the observation of an effect. In this work, we present the development of molecular tools for both of these aspects. The first chapter highlights our rational development of a photo-controlled formaldehyde do-nor with an internal fluorescence response upon activation. This tool enabled us to establish an assay for the quantification of the released reactive carbon species into the intracellular space. Using this unique approach, we evaluated the dose-dependent impairment of HEK293 cells by formaldehyde in a wound healing assay and showed the potential cytotoxicity of common formaldehyde-releasing prodrug approaches. The second and third chapter summarize our ap-proaches towards activatable contrast agents for photoacoustic imaging, an emerging deep-tissue imaging modality. To address a lack of modular activatable probes for this imaging technique, we combined the analyte specificity of DNA aptamers with commercially available fluorophores to show the feasibility of a general approach. For our proof-of-concept study, we used a thrombin-selective aptamer and were able to show a selective modulation of the photo-acoustic signal upon exposure to thrombin in vitro and in vivo. In addition, we are currently developing new dye platforms, imaging techniques and probes for photoacoustic imaging.
Graduation Semester
2020-05
Type of Resource
Thesis
Permalink
http://hdl.handle.net/2142/108239
Copyright and License Information
Copyright 2020 Lukas P. Smaga

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