University of Illinois Urbana-Champaign

Defluorination of PFOA and PFOS alters their toxicity in a hepatocyte and hepatic stellate cell co-culture system

Wen, Yi

Content Files
Wen_Yi_ECEC21.pdf

Permalink

https://hdl.handle.net/2142/109912

Description

Title
Defluorination of PFOA and PFOS alters their toxicity in a hepatocyte and hepatic stellate cell co-culture system
Author(s)
Wen, Yi
Issue Date
2021-04-27
Keyword(s)
  • per- and polyfluoroalkyl substances
  • PFAS
Date of Ingest
2021-04-26T16:55:47Z
Abstract
Presented by: Yi Wen – Graduate Student at University of Illinois at Urbana-Champaign, yiwen4@illinois.edu Co-authors: Saeed Ahmad, Hyeon Ryoo, Zekun Liu, Jinyong Liu, Gregory H. Underhill, Joseph Irudayaraj Abstract: Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) are two of the most widely used fluorosurfactants in the global industry. They are extremely persistent in the environment and have long half-life in humans. Recent efforts have been made to degrade or defluorinate long-chain perfluoroalkyl substances (PFAS) to reduce their toxicity. But little biological validation has been performed to demonstrate the reduction in toxicity of these defluorinated chemicals. A co-culture system of human hepatic stellate cells and spheroids formed of hepatocellular carcinoma cell HepG2 was designed to mimic human liver in vitro. This system was used as a model to study the toxicity of defluorinated PFOA and PFOS versus their intact forms. Both types of cells are evaluated on the different aspects of the PFAS toxicity, including cytotoxicity, metabolic toxicity, and epigenetic toxicity. Biography: Yi is a Ph.D. candidate in Dr. Joseph Irudayaraj Lab. He holds a M.S. in Bioengineering from University of Illinois as well as a B.S. in Biological Engineering and a B.S. in Biochemistry from Purdue University. He believes in translating the cutting-edge biology knowledge into tools to explore and reverse the epigenetic toxicity effects of environmental toxicants. He also works on targeted drug delivery with nanoparticles. Yi also has passion in teaching the next generation of bioengineers. Photochemical fate of quinone outside inhibitor fungicides
Series/Report Name or Number
2021 Emerging Contaminants in the Environment Conference (ECEC21)
Type of Resource
  • text
  • still image
Genre of Resource
  • presentation/lecture/speech
  • conference paper
Language
en
Permalink
  • http://hdl.handle.net/2142/109912
  • https://youtu.be/VVfEz3XeQbI

Owning Collections

2021 Emerging Contaminants in the Environment Conference (ECEC21) PRIMARY