University of Illinois Urbana-Champaign

Neonatal Mouse Ovary Culture Incorporated in Cisplatin Treatment Using the Hanging Drop Method

Joo, Erica

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https://hdl.handle.net/2142/114213

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Title
Neonatal Mouse Ovary Culture Incorporated in Cisplatin Treatment Using the Hanging Drop Method
Author(s)
Joo, Erica
Contributor(s)
Qiao, Joe (Huanyu)
Issue Date
2022
Keyword(s)
Comparative Biosciences
Date of Ingest
2022-06-01T16:50:53Z
Abstract
My research focuses on BLM helicase’s role throughout meiotic recombination. BLM helicase is an enzyme that unwinds DNA and contributes to DNA repair mechanisms. BLM is a part of the RecQ helicase family, and a mutation of the BLM gene causes Bloom’s Syndrome - characterized by premature aging and/or a predisposition to cancer. In order to visualize BLM helicase’s role during the earlier stages of meiosis, I conduct mice dissections on neonatal (newborn) mice that are 1-2 days old. Using a petri dish, I would hang a drop of ovary culture media onto the lid and insert an ovary into the drop. Placing the lid on the dish, the “hanging drop” helps promote more treatment into the sample. I am doing a 48-hour culture using the hanging-drop method with/without a range of cisplatin concentrations, then using immunolabeling to see if the control group induces intensive gamma-H2AX staining. Further insight into BLM can expand our knowledge in the mechanisms driving these meiotic pathways and ultimately infertility, miscarriage, birth defects, and cancers. This image visualizes the mouse ovary (center white dot) in a drop of ovary culture medium (pink) with an additional cisplatin concentration.
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  • Image
Language
eng
Permalink
http://hdl.handle.net/2142/114213
Copyright and License Information
Copyright 2022 Erica Joo

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