Biological methane conversion by methanotrophic bacteria
Pei, Sichong
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https://hdl.handle.net/2142/117769
Description
Title
Biological methane conversion by methanotrophic bacteria
Author(s)
Pei, Sichong
Issue Date
2022-11-28
Director of Research (if dissertation) or Advisor (if thesis)
Rao, Christopher V
Doctoral Committee Chair(s)
Rao, Christopher V
Committee Member(s)
Leckband, Deborah E
Shukla, Diwakar
Jensen, Paul A
Department of Study
Chemical & Biomolecular Engr
Discipline
Chemical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Methanotroph
Methane Oxidation
Language
eng
Abstract
In this dissertation, research works were presented on exploration of different aspects in biological methane conversion by methanotrophic bacteria using both experimental and computational techniques. First, robustness of methanotroph Methylococcus capsulatus Bath in raw natural gas was examined. Significant amount of hydrogen sulfide may exist in raw natural gas, and studies on its stress response toward toxicity of hydrogen sulfide remains unknown. An extensive investigation on viability of methanotroph in raw natural gas will improve its commercialization progress. Using next-generation sequencing technique and bioinformatic analysis, the transcriptomic change of M. capsulatus Bath toward toxicity of hydrogen sulfide was profiled at molecular level and it revealed unexpected observations. Although the presence of hydrogen sulfide slows the growth of M. capsulatus Bath, sulfide was shown to upregulate sulfide oxidation related enzymes, sulfide quinone reductase and persulfide dioxygenase as well as a novel lanthanide-dependent methanol dehydrogenase. Furthermore, efforts have been made on investigation of a feasible methane bioreactor. An inhouse bench-scale airlift column reactor was designed and constructed where methane conversion performance was studied. At last, the manuscript of an earlier cooperative publication on chemotaxis of Bacillus subtilis was included.
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