Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples
Heredia, John
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https://hdl.handle.net/2142/124434
Description
Title
Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples
Author(s)
Heredia, John
Issue Date
2024-05-02
Director of Research (if dissertation) or Advisor (if thesis)
Valera, Enrique
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Microfluidics
Blood
Crp
Creactiveprotein
Sepsis
Labonachip
Optical
Fluorescence
Language
eng
Abstract
Sepsis is a life-threatening condition with a high healthcare burden in terms of patient death and hospital readmissions. Despite its cost, sepsis remains difficult to treat due to days-long layover for lab testing as well as its nonspecific symptoms. In recent years, multiple technologies have been developed capable of detecting biomarkers of sepsis. Some technologies detect circulating proteins, others detect proteins expressed on the surface of cells (cell-surface proteins). However, detecting both types of biomarkers from a whole blood sample remains challenging. Here we propose a technology capable of doing this by combining specific capture, immunoassays and optical detection. In this Master's Thesis we have developed the protocol for the detection of C-Reactive Protein (CRP) from whole blood samples using a sandwich immunoassay and fluorescent microspheres. Additionally, we have developed the microscope setup for optical detection and demonstrated on-chip CRP detection from whole blood samples.
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