University of Illinois Urbana-Champaign

Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples

Heredia, John

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https://hdl.handle.net/2142/124434

Description

Title
Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples
Author(s)
Heredia, John
Issue Date
2024-05-02
Director of Research (if dissertation) or Advisor (if thesis)
Valera, Enrique
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
  • microfluidics
  • blood
  • crp
  • creactiveprotein
  • sepsis
  • labonachip
  • optical
  • fluorescence
Abstract
Sepsis is a life-threatening condition with a high healthcare burden in terms of patient death and hospital readmissions. Despite its cost, sepsis remains difficult to treat due to days-long layover for lab testing as well as its nonspecific symptoms. In recent years, multiple technologies have been developed capable of detecting biomarkers of sepsis. Some technologies detect circulating proteins, others detect proteins expressed on the surface of cells (cell-surface proteins). However, detecting both types of biomarkers from a whole blood sample remains challenging. Here we propose a technology capable of doing this by combining specific capture, immunoassays and optical detection. In this Master's Thesis we have developed the protocol for the detection of C-Reactive Protein (CRP) from whole blood samples using a sandwich immunoassay and fluorescent microspheres. Additionally, we have developed the microscope setup for optical detection and demonstrated on-chip CRP detection from whole blood samples.
Graduation Semester
2024-05
Type of Resource
Thesis
Copyright and License Information
Copyright 2024 John Heredia

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