HOLOGRAPHIC FAST GRADIENT LIGHT INTERFERENCE MICROSCOPY (HF-GLIM)

Sakakura, Masayoshi

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https://hdl.handle.net/2142/125064 Copy

Description

Title

HOLOGRAPHIC FAST GRADIENT LIGHT INTERFERENCE MICROSCOPY (HF-GLIM)

Author(s)

Sakakura, Masayoshi

Issue Date

2020-05-01

Keyword(s)

Holography; Quantitative phase imaging; Sagnac interferometer; Gradient light interference microscopy; Differential interference contrast microscopy

Date of Ingest

2024-11-08T10:24:27-06:00

Abstract

Quantitative phase imaging has been an emerging technique in the field of biomedical imaging. As one type, gradient light interference microscopy (GLIM) is used to retrieve a quantitative gradient mapping of the phase delay that is produced by a biological sample. The method involves collecting four phaseshifted frames for a single field of view, which ultimately combines arithmetically into phase gradient information. The phase- shifting is done by a space light modulator (SLM) which intakes an appropriate voltage that converts into a corresponding phase retardation in the image field. Despite GLIM’s ability to generate a quantitative phase gradient of a sample, such module depends heavily on the operational speed of the SLM. The holographic fast gradient light interference microscopy (HF-GLIM) aims to retrieve a field of view with only a single image from the conventional differential interference contrast microscope (DIC), and is sent to a tilted Sagnac interferometer to create a holographic output at the camera. Through digital processing of the Hologram using Fourier filtering, the phase gradient that is given by the sample can be retrieved only through a single recording instance from the HF-GLIM.

Type of Resource

text

Genre of Resource

dissertation/thesis

Language

eng

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