Circadian rhythmicity of rat neuroendothelial cells at the blood-brain interface

Weiss, Amanda C

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https://hdl.handle.net/2142/125600 Copy

Description

Title

Circadian rhythmicity of rat neuroendothelial cells at the blood-brain interface

Author(s)

Weiss, Amanda C

Issue Date

2024-07-11

Director of Research (if dissertation) or Advisor (if thesis)

Gillette, Martha U

Doctoral Committee Chair(s)

Gillette, Martha U

Committee Member(s)

• Kong, Hyunjoon
• Chung, Hee Jung
• Sweedler, Jonathan V

Department of Study

Molecular & Integrative Physl

Discipline

Molecular & Integrative Physi

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• blood-brain barrier
• circadian rhythms
• blood-brain interface
• blood-brain barrier permeability

Abstract

The Blood-Brain Interface (BBI) facilitates neurological protection from pathogens, the removal of waste products, brain homeostasis, and the permeation of nutrients and drugs. Its dysfunction contributes to the onset of a variety of neurological diseases, including strokes and dementia. Research has shown that both BBI permeability and human susceptibility to strokes fluctuate according to time of day. However, more in vitro studies are required to isolate the effects of circadian rhythms from the effects of sleep and other confounding factors on BBI permeability. The role of neuroendothelial cells, a key component of the BBI, has also not been fully elucidated. We have determined that barrier tightness and permeability oscillate according to time of day in rat neuroendothelial cells. These findings extended to permeability to the blood clotting factor fibrinogen. We also determined that the addition of Carbachol, a pan-acetylcholine receptor agonist, could shift these rhythms.

Graduation Semester

2024-08

Type of Resource

Thesis

Handle URL

https://hdl.handle.net/2142/125600

Copyright and License Information

Copyright 2024 Amanda Weiss

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