Hydrothermal synthesis and characterization of carbon dots for enhanced biosensing: A focus on optical properties and DNA interactions
Morshed, Mehzabin
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https://hdl.handle.net/2142/127358
Description
Title
Hydrothermal synthesis and characterization of carbon dots for enhanced biosensing: A focus on optical properties and DNA interactions
Author(s)
Morshed, Mehzabin
Issue Date
2024-11-22
Director of Research (if dissertation) or Advisor (if thesis)
Wang, Xing
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Carbon dots (CDs)
Biosensing applications
Fluorescence quenching
Optical properties
Nucleic acid interactions
Point-of-care diagnostics
Real-time biosensing
Carbon precursor optimization
Functionalization agents
Viral detection
DNA structure-dependent quenching
Abstract
Carbon dots (CDs) have emerged as a promising class of nanomaterials for biosensing applications due to their unique optical properties, including high fluorescence quantum yields, photostability, and biocompatibility. This thesis investigates the synthesis, characterization, and application of CDs in biosensing, focusing on the fluorescence quenching phenomena associated with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The synthesis of CDs was optimized using various carbon precursors and functionalization agents to enhance their fluorescence properties. The interaction dynamics between CDs and nucleic acids were analyzed through concentration, time and structure dependent studies, revealing that these parameters significantly affect fluorescence intensities with quenching effects. The study further explores possible utilization of CDs in real-time biosensing, highlighting their potential for use in point- of-care diagnostics. Findings indicate that the CDs can effectively bind to nucleic acids while maintaining fluorescence, suggesting their versatility in detecting various molecular targets. This research contributes to the understanding of the behavior of CDs in biological systems and underscores their significance as effective fluorescent probes for viral detection.
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