Label-free polarization-sensitive multimodal optical microscopy for collagen characterization in tissue
Yang, Lingxiao
This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.
Permalink
https://hdl.handle.net/2142/127390
Description
Title
Label-free polarization-sensitive multimodal optical microscopy for collagen characterization in tissue
Author(s)
Yang, Lingxiao
Issue Date
2024-12-06
Director of Research (if dissertation) or Advisor (if thesis)
Boppart, Stephen A
Doctoral Committee Chair(s)
Boppart, Stephen A
Committee Member(s)
Bhargava, Rohit
Dragic, Peter D
Zhao, Yang
Department of Study
Electrical & Computer Eng
Discipline
Electrical & Computer Engr
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
optical imaging, biomedical optics, collagen
Abstract
This thesis presents the development and application of a novel multimodal polarization-sensitive optical microscopy system for comprehensive characterization of collagen in biological tissues, with a particular focus on tumor microenvironments. The work addresses the critical need for advanced imaging techniques to study collagen beyond morphological features, offering unprecedented insights into its structural, biophysical and biochemical properties across multiple scales.
The thesis first introduces the important role of collagen in the extracellular matrix and tumor progression, and the concept of tumor-associated collagen signatures (TACS). Details on the methodology of the custom-built multimodal imaging system are then described, integrating polarization-sensitive second harmonic generation (PSHG), polarization-sensitive optical coherence microscopy (PSOCM), two-photon autofluorescence lifetime imaging microscopy (2PFLIM), and coherent anti-Stokes Raman scattering (CARS) microscopy. Key technical innovations include the design and calibration of a custom Fourier transform pulse shaper for low-photodamage CARS are highlighted.
The system's capabilities are first demonstrated through the characterization of different collagen types (I-IV) in purified gels and complex tissue environments. Distinct optical signatures are identified for each collagen type, and the integration of PSOCM measurements into the PSHG numerical model significantly improves collagen helix angle estimations in thick tissues. A comprehensive analysis of collagen organization in rodent outer ears reveals depth-dependent distributions of different collagen types and enables identification of complex structures.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
