Developing a medium-throughput collagen biomaterial model system
Rubino, Grace Alexandra
This item's files can only be accessed by the System Administrators group.
Permalink
https://hdl.handle.net/2142/127477
Description
Title
Developing a medium-throughput collagen biomaterial model system
Author(s)
Rubino, Grace Alexandra
Issue Date
2024-12-11
Director of Research (if dissertation) or Advisor (if thesis)
Harley, Brendan
Department of Study
Chemical & Biomolecular Engr
Discipline
Chemical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Bone Regeneration
Collagen Scaffold
Hydrogel Biomaterial
Language
eng
Abstract
The field of biomedical research has long recognized the need to develop higher order biomaterial model systems for improved disease characterization and translational therapeutic/material progress. There is, however, difficulty in developing these workflows at the scale of conventional two-dimensional cell culture screening systems while simultaneously approaching a level of complexity necessary to consider translation to in vivo animal models. Here, we describe a three-dimensional (3D), in vitro model system to investigate the impact of stromal cell migration from one microenvironment to another at a medium-throughput scale. Importantly, we demonstrate the ability of this workflow to be utilized as a screening tool for collagen-based biomaterial motifs of interest in promoting craniomaxillofacial bone defect repair. As potential next steps in utilization of this model, we probe questions underlying a dual modification of the mechanics and chemistry of a mesh collagen scaffold design for instructing bone regeneration, potential of a collagen scaffold to be drug loaded and quantifying a release profile, and lastly investigate collagen scaffolds in a new disease and cell context for understanding fibroblast mechanobiology. These serve as a preliminary set of materials designs for potential integration into the developed model system. Overall, we explore new routes of creating medium-throughput screening workflows and illuminate new material motifs that inform fundamental and translational biologic questions.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
