University of Illinois Urbana-Champaign

Evolution of transmission mode in a temperate virus of Pseudomonas aeruginosa and its effects on the host

Suttenfield, Laura Catherine

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Description

Title
Evolution of transmission mode in a temperate virus of Pseudomonas aeruginosa and its effects on the host
Author(s)
Suttenfield, Laura Catherine
Issue Date
2025-03-06
Director of Research (if dissertation) or Advisor (if thesis)
Whitaker, Rachel J
Doctoral Committee Chair(s)
Whitaker, Rachel J
Committee Member(s)
  • Slauch, James M
  • Kuzminov, Andrei
  • Vanderpool, Carin
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • lysogen
  • virus
  • transposable phage
  • DMS3
  • Pseudomonas aeruginosa
  • vertical transmission
Abstract
Bacteria are known for the infections they produce; that they are themselves infected is not often considered. This thesis considers the latent, lysogenic phase of a temperate virus DMS3 of the opportunistic pathogen Pseudomonas aeruginosa. In Chapter 1, I review the existing literature on how lysogeny contributes to viral fitness. In Chapter 2, we attempt to test this with deep sequencing of semi-random insertion sites. In Chapter 3, we find that spontaneous induction in lysogens causes divergent evolutionary resolutions. In Chapter 4, we take this approach to whole populations to test the way that infected and uninfected P. aeruginosa evolves in the presence of an antibiotic which can induce the lysogen. We find substantial evidence that this temperate virus impacts the evolution of its pathogen host.
Graduation Semester
2025-05
Type of Resource
Thesis
Handle URL
https://hdl.handle.net/2142/129496
Copyright and License Information
Copyright 2025 Laura Suttenfield

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