Rooting for ecosystem services through haplotype analysis
Livingston, Abby
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https://hdl.handle.net/2142/130584
Description
Title
Rooting for ecosystem services through haplotype analysis
Author(s)
Livingston, Abby
Contributor(s)
Bohn, Martin
Godoy Dos Santos, Jenifer
Ugarte, Carmen
Issue Date
2025-11-12
Keyword(s)
haplotypes
roots
maize
root complexity
genetics
corn
maize genetics
Date of Ingest
2025-12-15T11:45:47-06:00
Abstract
Organic farming systems present a suite of biotic and abiotic challenges, including intense weed competition, pest pressure, and disease prevalence. Genotypic variation among maize cultivars plays a critical role in crop performance under these conditions. The root system, as the primary interface with the rhizosphere, has the potential to enhance ecosystem services important for productivity in organic systems. This project aims to identify root-associated haplotypes in maize that contribute to favorable rhizosphere functioning. We developed the Elite Maize Association Mapping Panel (EMAMP), consisting of over 1,650 recombinant inbred lines (RILs) derived from 66 elite F1 hybrids. All RILs were genotyped using the MaizeSNP50 array. Haplotype blocks will be extracted, converted into multiallelic markers, and used to define root haplotype clusters. RILs from the same haplotype cluster but with diverse genetic backgrounds will be crossed to generate hybrids. These inbreds and hybrids will be evaluated in organic field trials for root architecture and agronomic performance. Our goal is to identify root haplotypes that promote ecosystem services such as improved nutrient cycling, weed suppression, and stress resilience. This poster presents our genomic pipeline for haplotype identification and preliminary insights into haplotype frequency and potential function. Ultimately, this work supports the development of regionally adapted root ideotypes tailored to the needs of organic maize production.
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