University of Illinois Urbana-Champaign

Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus

Jilka, Joseph M.

This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.

Permalink

https://hdl.handle.net/2142/21792

Description

Title
Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus
Author(s)
Jilka, Joseph M.
Issue Date
1990
Doctoral Committee Chair(s)
Clark, John M., Jr.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2011-05-07T13:19:16Z
Keyword(s)
  • Biology, Molecular
  • Agriculture, Plant Pathology
  • Chemistry, Biochemistry
Language
eng
Abstract
Double-stranded cDNAs derived from the 3$\sp\prime$ terminus of maize dwarf mosaic virus, strain A (MDMV-A), maize dwarf mosaic virus, strain B (MDMV-B), maize dwarf mosaic virus, strain KS1 (MDMV-KS1), maize dwarf mosaic virus, strain O (MDMV-O), and sugarcane mosaic virus strain H have been cloned as a first step in the creation of transgenic MDMV and SCMV resistant maize plants. These cDNA clones contain cDNA inserts derived from varying lengths of the coding region of the nuclear inclusion II protein, the complete coat protein cistron, and the complete untranslated region of these viruses plus a poly-adenylated 3$\sp\prime$-terminal tail. Nucleotide sequence analyses of each of these cDNA inserts have been determined. Homology studies carried out on the resultant ds DNA sequences and derived amino acid sequences show regions of high homology in comparison to one another and to previously published potyvirus nuclear inclusion II and coat protein amino acid sequences. Amino acid homologies of the derived amino acid sequences show the N-termini of the coat proteins of these viruses exhibit considerable variability and support immunological data establishing four distinct groups within the MDMV and SCMV viruses. Homology studies identified the site of proteolytic cleavage between the nuclear inclusion II proteins and the coat proteins of these viruses. Additionally, oligodeoxynucleotide directed site specific mutagenesis alteration of the MDMV-B cDNA clone allows excision of the NI$\sb{\rm II}$ coding region and introduction of a translation start site for the (MDMV-B) coat protein. Subsequent in vitro transcription and translation yields immunologically distinct MDMV-B coat protein. This thesis presents the first step in the potential generation of transgenic maize plants that express MDMV and SCMV coat proteins and exhibit resistance to infection by various strains of MDMV and SCMV.
Type of Resource
text
Permalink
http://hdl.handle.net/2142/21792
Copyright and License Information
Copyright 1990 Jilka, Joseph M.

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Biochemistry