Adhesion and intercellular junction formation by pulmonary intravascular macrophages and endothelial cells in vitro
Morton, Daniel George
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Permalink
https://hdl.handle.net/2142/23465
Description
Title
Adhesion and intercellular junction formation by pulmonary intravascular macrophages and endothelial cells in vitro
Author(s)
Morton, Daniel George
Issue Date
1990
Department of Study
Pathobiology
Discipline
Pathobiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Veterinary Science
Language
eng
Abstract
Porcine pulmonary intravascular macrophages were isolated from six to eight week old pigs by perfusing the pulmonary vasculature with collagenase solution. The isolated cells were nonspecific esterase positive, phagocytic, and had morphologic features of macrophages. Isolated intravascular macrophages adhered in greater numbers to cultured porcine endothelial cells than to porcine fibroblasts, PK-15 porcine kidney epithelial cells, plastic, or basement membrane material. In contrast, alveolar macrophages adhered in greater numbers to plastic surfaces than to any of the cellular substrates. Adherence of intravascular macrophages to endothelial cells was inhibited by coculturing intravascular macrophages with indomethacin or pretreating intravascular macrophages with indomethacin before coculturing the cells with endothelium. Pretreatment of endothelial cells with indomethacin did not inhibit adherence of intravascular macrophages to endothelium. These results suggest that cyclooxygenase products of arachidonic acid from intravascular macrophages are important in modulating adherence of intravascular macrophages or their precursors to endothelium. A23187 added to cocultures enhanced adherence of intravascular macrophages to plastic, but not to endothelium. Pretreatment of either cell type with A23187 did not alter detectably adherence of intravascular macrophages to endothelial cells. Treatment of intravascular macrophages or endothelial cells with bacterial lipopolysaccharide and dexamethasone did not inhibit or stimulate adhesion of intravascular macrophages to endothelium. Intravascular macrophages incubated with aortic and pulmonary arterial explants for four or eight hours formed intercellular adhesion plaques in vitro.
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