Comparative osteogenesis of equine mesenchymal stem cells isolated from bone marrow, adipose tissue, and synovium

Pantaleoni Andrietti, Antonella

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https://hdl.handle.net/2142/31213 Copy

Description

Title

Comparative osteogenesis of equine mesenchymal stem cells isolated from bone marrow, adipose tissue, and synovium

Author(s)

Pantaleoni Andrietti, Antonella

Issue Date

2012-05-22T00:36:01Z

Director of Research (if dissertation) or Advisor (if thesis)

Stewart, Matthew C.

Department of Study

Vet Clinical Medicine

Discipline

VMS-Veterinary Clinical Medcne

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• equine mesenchymal stem cells
• bone marrow derived mesenchymal stem cells
• synovium derived mesenchymal stem cells
• adipose tissue derived mesenchymal stem cells
• osteogenesis

Abstract

The experiments in this study were carried out to define the osteogenic potential of equine MSCs derived from synovium, bone marrow, and adipose tissue maintained under the same culture conditions. Equine MSCs were isolated from synovium [SYN], bone marrow [BM] and adipose tissue [AD], expanded in monolayer culture for two passages, and then transferred to basal or standard osteogenic medium. At day 7 and 14, the phenotypic status of the cells was primarily evaluated by staining for mineralized matrix (Alizarin Red and Von Kossa) and the enzymatic activity of alkaline phosphatase (ALP). Other secondary indicators of osteogenic differentiation included in this study were mRNA levels of ALP and osteoblast-specific genes Runx2, Osterix, Osteonectin and Osteomodulin. Staining of the mineralized matrix of BM-MSCs cultured under osteogenic conditions, demonstrated a higher uptake of both Alizarin Red (AR) and Von Kossa (VK) stains, restricted to dense cellular aggregation (nodules). Stain up-take in SYN and AD cultures was also limited to infrequent sites of cellular aggregation but was less intense than in BM cultures. In osteogenic cultures, intense ALP activity was present within and immediately around the cell aggregates in BM cultures, whereas staining in the SYN and AD monolayers was far less intense and more diffusely distributed. BM-MSCs cultured under osteogenic conditions increased ALP mRNA expression and ALP activity. Osteogenic AD cultures also significantly increased ALP activity by day 14. In SYN cultures, basal ALP activity was comparatively low and, even under osteogenic conditions, there were negligible changes in ALP activity. These findings suggest that BM-MSCs are more able to undergo osteogenic differentiation than SYN- or AD-MSCs. Bone marrow-derived cells should be preferred for any application focused on bone regeneration.

Graduation Semester

2012-05

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http://hdl.handle.net/2142/31213

Copyright and License Information

Copyright 2012 Antonella Pantaleoni Andrietti

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