Amino Acid Sequence Studies on the Beta Subunit of Bacterial Luciferase
Thompson, Richard Blair
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https://hdl.handle.net/2142/70502
Description
Title
Amino Acid Sequence Studies on the Beta Subunit of Bacterial Luciferase
Author(s)
Thompson, Richard Blair
Issue Date
1981
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Abstract
Peptides comprising 80% of the amino acid sequence of the (beta) subunit of bacterial luciferase from Vibrio harveyi were isolated and their amino acid sequences were determined by Edman degradation. The protein was fragmented by digestion with S. aureus V8 protease, trypsin, trypsin after citraconylation, and by treatment with cyanogen bromide. A substantial proportion of each peptide mixture precipitated and was not amenable to separation. Peptides were isolated by gel filbration, ion exchange chromatography, paper electrophoresis, and high performance reverse phase liquid chromatography. We have found no significant similarity between the (alpha) and (beta) subunits beyond their N-termini. We suggest that the N-terminal similarity may have some role in the function of the protein. Moreover, the (beta) subunit displays no sequence similarity to other flavoproteins, including regions and residues implicated in flavin binding by these enzymes. Therefore, our data do not suggest a role for the (beta) subunit in binding reduced flavin mononucleotide, but they do support the view that luciferase is significantly different from all other flavin monoxygenases.
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