Evaluation of Methods for Detecting Coliforms and Fecal Streptococci in Chlorinated Sewage Effluents

Lin, S.D.

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https://hdl.handle.net/2142/77788 Copy

Description

Title

Evaluation of Methods for Detecting Coliforms and Fecal Streptococci in Chlorinated Sewage Effluents

Author(s)

Lin, S.D.

Issue Date

1974

Keyword(s)

• bacteria
• chlorination
• enrichment procedure
• environmental engineering
• fecal coliforms
• fecal streptococci
• MF and MPN methods
• prolonged incubation
• sanitary engineering
• secondary sewage effluents
• total coliforms
• water polution

Abstract

Total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) recoveries in chlorinated secondary sewage effluents were investigated by the membrane filter (MF) and multiple-tube (most probable number, MPN) methods. The LES two-step MF method was found to be comparable to the MPN procedure for determining TC. The TC detection was 1.5 times greater by the LES two-step technique than that obtained by the M-Endo one-step MF procedure. Fecal coliform recovery by the M-FC MF procedure was lower than the recovery obtained by the MPN method. Azide-dextrose broth, brain-heart infusion broth, and peptone yeast-extract casitone used separately with the M-Enterococcus agar MF2 (2-day incubation) procedure were not satisfactory for the recovery of FS. The M-Enterococcus agar procedure with bile broth enrichment (MF2) or prolonged incubation for 3 days (MF3) significantly increased FS recovery and both were comparable to the MPN method. The results cited should be useful in assessing the efficiency of disinfection practices for waste treatment plants employing effluent chlorination.

Publisher

Illinois State Water Survey

Series/Report Name or Number

Report of Investigation (Illinois State Water Survey) RI-078

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/77788

Copyright and License Information

Copyright, University of Illinois Board of Trustees. All rights reserved. This document is a product of the Illinois State Water Survey, and has been selected and made available by the Illinois State Water Survey and the University Library, University of Illinois at Urbana-Champaign. It is intended for research and educational use, and proper attribution is requested.

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