University of Illinois Urbana-Champaign

Hydrolysis and Toxicity of Aspartyl Peptides and the Influence of Metal Ions on Aspartyl Peptide Hydrolases in Salmonella Enterica Serovar Typhimurium

Broder, Daniel Howard

Content Files
3131065.pdf

Permalink

https://hdl.handle.net/2142/86668

Description

Title
Hydrolysis and Toxicity of Aspartyl Peptides and the Influence of Metal Ions on Aspartyl Peptide Hydrolases in Salmonella Enterica Serovar Typhimurium
Author(s)
Broder, Daniel Howard
Issue Date
2003
Doctoral Committee Chair(s)
Miller, Charles G.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2015-09-28T15:17:18Z
Keyword(s)
Chemistry, Biochemistry
Language
eng
Abstract
Extracts of a multiply peptidase deficient (MPD)(pepN,A,B,D,P,Q,T,E,iadA,iaaA ) Salmonella enterica serovar Typhimurium strain contain an aspartyl dipeptidase activity which is dependent on Mn2+. Purification of this activity followed by N-terminal sequencing of the protein suggested that the Mn2+-dependent peptidase is DapE, N-succinyl-L,L-diaminopimelate desuccinylase. Disruption of the dapE gene and transfer of the mutation into a MPD strain yielded a strain with extract inactive towards Asp-Leu or any other peptide tested. This strain would not utilize any peptides tested as amino acid sources. Purified DapE also exhibited Mn2+-dependent activity towards aspartyl peptides and specificity studies with pure enzyme revealed it was specific for aspartyl dipeptides. Metal binding experiments indicated that, of two metal binding sites of unequal affinity in DapE, Mn 2+ is bound to the low affinity site and Zn2+ to the high affinity site when DapE functions as a peptidase. Overexpression of DapE in the MPD dapE strain conferred strong growth on Asp-Leu as a Leu source indicating DapE functions well as a peptidase when overexpressed. Aspartyl peptides were demonstrated to be toxic to the MPD dapE strain, but not to a wild type peptidase strain. This bacteriocidal effect could be antagonized by reintroduction of peptidases specific for aspartyl peptides. Growth of MPD dapE in the absence of aspartyl peptides was very slow, but could be enhanced by the introduction of aspartyl peptidases suggesting that internally generated toxic aspartyl peptides cause slow growth of peptidase deficient strains. Overproduction of aspartyl dipeptide specific peptidases nearly completely rescued slow growth of MPD dapE. Transduction of MPD dapE and selection for growth on Asp-Leu as a Leu source revealed pepD, pepA, pepE, and pepB . Other strains containing a single peptidase gene were constructed. Extracts from the pepA+, pepB +, pepD+, and dapE + strains all showed activation by Mn2+ for aspartyl peptidase activity. Disruption of Mn2+ transport dramatically affected aspartyl peptide utilization by pepA+, pepD+, and dapE+-strains, indicating that these enzymes all utilize Mn2+ in vivo for aspartyl peptidase activity. The implications of Mn2+ activation of peptidases and the potential variability of peptidase bound cations in vivo are discussed.
Graduation Semester
2003
Type of Resource
text
Permalink
http://hdl.handle.net/2142/86668

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Microbiology