Chromosomal Fragmentation in Escherichia Coli: Its Absence in mutT Mutants and Its Mechanisms in seqA Mutants
Rotman, Ella Rose
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https://hdl.handle.net/2142/86712
Description
Title
Chromosomal Fragmentation in Escherichia Coli: Its Absence in mutT Mutants and Its Mechanisms in seqA Mutants
Author(s)
Rotman, Ella Rose
Issue Date
2009
Doctoral Committee Chair(s)
Kuzminov, Andrei
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Language
eng
Abstract
While studying mutT and seqA mutants, we discovered a contaminating bacteriophage We studied, sequenced, and identified the phage as phi80. We looked at three interesting phenotypes: modification of the phage DNA to protect against restriction enzymes, sensitivity of lysogens to UV damage, and the toxicity when the gam gene was overexpressed in the cell.
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