Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome
Rohloff, Peter John
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https://hdl.handle.net/2142/87623
Description
Title
Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome
Author(s)
Rohloff, Peter John
Issue Date
2003
Doctoral Committee Chair(s)
Roberto Docampo
Department of Study
Veterinary Pathobiology
Discipline
Veterinary Pathobiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Health Sciences, Pathology
Language
eng
Abstract
Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes trafficked toward the contractile vacuole during hyposmotic stress in a cAMP- and microtubule-dependent fashion. This phenomenon was functionally significant, since aquaporin and microtubule inhibitors reduced the rate of volume recovery. Isolated contractile vacuoles were enriched in alkaline phosphatase, aquaporin, and some acidocalcisomal markers.
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