Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy & Single Molecule Nano-Metronome & Single Molecule Study of Tetramethyrhodamine -Dna Interaction
Buranachai, Chittanon
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Permalink
https://hdl.handle.net/2142/87911
Description
Title
Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy & Single Molecule Nano-Metronome & Single Molecule Study of Tetramethyrhodamine -Dna Interaction
Author(s)
Buranachai, Chittanon
Issue Date
2008
Doctoral Committee Chair(s)
Clegg, Robert M.
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, General
Language
eng
Abstract
The final part presents the single molecule study on a behavior of a popular fluorescent dye called Tetramethylrhodamine (TMR) attached to the end of DNA oligomer. The study reveals that in the absence of any biological activities of DNA, the fluorescence emission of TMR undergoes multiple slow transitions among at least four different fluorescence states. While the actual number of fluorescence states and therefore the actual rates of transition among them remains open for discussion and more control measurements are definitely required, we are able to show that each fluorescence state lasts on the average in a range of hundreds of milliseconds to several seconds. These long dwell times are not observable in most of the studies done on freely diffusing TMR-DNA conjugate. It is unlikely that the fluctuation originates from the non-specific interactions between the dye and the glass surface and the interaction appears to be specific to TMR-DNA system. Therefore, TMR should be used with caution when conjugated to DNA.
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