Femtosecond pulse shaping for enhanced optical imaging and control of cells
Ark, Eugene D
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https://hdl.handle.net/2142/89209
Description
Title
Femtosecond pulse shaping for enhanced optical imaging and control of cells
Author(s)
Ark, Eugene D
Issue Date
2015-11-24
Director of Research (if dissertation) or Advisor (if thesis)
Boppart, Stephen A.
Department of Study
Electrical & Computer Engineering
Discipline
Electrical & Computer Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Femtosecond pulse shaping
caged compound
opsin
Abstract
Optical techniques have garnered increasing interest in biomedical research. As the types of optical probes available continue to expand, the appeal of experiments utilizing multiple probes simultaneously grows rapidly. Unfortunately, the significant spectral overlap in probe responsiveness presents limitations on their potential applications. Much of the effort in circumventing this issue has been focused on developing modified probes with shifted spectral sensitivities; however, this is a long and inefficient process that tends to have undesired consequences on probe activity. This thesis explores the possibility of using temporal pulse shaping in order to improve the selectivity with which optically sensitive molecules can be activated. It is demonstrated that linearly chirped pulses and dark pulses have the potential to improve selectivity by exclusively controlling the spectral phase function of the illumination source for three classes of optical probes: fluorescent molecules, caged compounds, and opsins.
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