Utilization of microwave spectroscopy to identify and probe reaction dynamics of hsno, a crucial biological signaling molecule

Nava, Matthew

Permalink

https://hdl.handle.net/2142/91423 Copy

Description

Title

Utilization of microwave spectroscopy to identify and probe reaction dynamics of hsno, a crucial biological signaling molecule

Author(s)

Nava, Matthew

Contributor(s)

• McCarthy, Michael C.
• Cummins, Christopher
• Stanton, John F.
• Martin-Drumel, Marie-Aline

Issue Date

2016-06-22

Keyword(s)

Spectroscopy as an analytical tool

Date of Ingest

2017-01-26T21:37:11Z

Abstract

Thionitrous acid (HSNO), a potential key intermediate in biological signaling pathways, has been proposed to link NO and H$_{2}$S biochemistries. Its existence and stability in vivo, however, remain controversial. By means of Fourier-transform microwave spectroscopy, we establish that HSNO is spontaneously formed in high concentration when NO and H$_{2}$S gases are simply mixed at room temperature in the presence of metallic surfaces. Our measurements reveal that HSNO is formed with high efficiency by the reaction H$_{2}$S and N$_{2}$O$_{3}$ to produce HSNO and HNO$_{2}$, where N$_{2}$O$_{3}$ is a product of NO disproportionation. These studies also suggest that further reaction of HSNO with H$_{2}$S may form HNO and HSSH. The length of the S--N bond has been derived to high precision from isotopic studies, and is found to be unusually long, 1.84 \AA\ -- the longest S--N bond reported to date for an SNO compound. The present structural and reactivity investigations of this elusive molecule provide a firm fundation to better understand its physiological chemistry and propensity to undergo S--N bond homolysis in vivo.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Genre of Resource

Conference Paper / Presentation

Language

En

Permalink

http://hdl.handle.net/2142/91423

Copyright and License Information

Copyright 2016 by the authors

Owning Collections