Detection of in vitro s-nitrosylated compounds with cavity ring-down spectroscopy

Rad, Mary Lynn

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https://hdl.handle.net/2142/91345 Copy

Description

Title

Detection of in vitro s-nitrosylated compounds with cavity ring-down spectroscopy

Author(s)

Rad, Mary Lynn

Contributor(s)

• Lehmann, Kevin
• Gaston, Benjamin M.
• Mezher, Monique Michele

Issue Date

2016-06-22

Keyword(s)

Spectroscopy as an analytical tool

Abstract

Nitric oxide has been of strong biological interest for nearly 40 years due to its role in cardiovascular and nervous signaling. It has been shown that S-nitrosocompounds are the main carrier molecule for nitric oxide in biological systems. These compounds are also of interest due to their relationship to several diseases including muscular dystrophy, stroke, myocardial infarction, Alzheimer's disease, Parkinson's disease, cystic fibrosis, asthma, and pulmonary arterial hypertension. Understanding the role of these S-nitrosocompounds in these diseases requires concentration studies in healthy and diseased tissues as well as metabolic studies using isotopically labeled S-nitroso precursors such at $^{15}$N-arginine. The current widely used techniques for these studies include chemiluminescence, which is blind to isotopic substitution, and mass spectrometry, which is known to artificially create and break S-NO bonds in the sample preparation stages. To this end we have designed and constructed a mid-IR cavity ring-down spectrometer for the detection of nitric oxide released from the target S-nitrosocompounds. Progress toward measuring S-NO groups in biological samples using the CRDS instrument will be presented.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

En

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http://hdl.handle.net/2142/91345

Copyright and License Information

Copyright 2016 by the authors

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