University of Illinois Urbana-Champaign

Biochemical characterization of five GH130-family enzymes from Caldanaerobius polysaccharolyticus ATCC BAA-17 and insights on their metabolic role and reaction mechanisms

Muller Paul, Hans M

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MULLERPAUL-THESIS-2016.pdf

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https://hdl.handle.net/2142/95627

Description

Title
Biochemical characterization of five GH130-family enzymes from Caldanaerobius polysaccharolyticus ATCC BAA-17 and insights on their metabolic role and reaction mechanisms
Author(s)
Muller Paul, Hans M
Issue Date
2016-12-09
Director of Research (if dissertation) or Advisor (if thesis)
Cann, Isaac
Department of Study
Animal Sciences
Discipline
Animal Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Date of Ingest
2017-03-01T17:02:12Z
Keyword(s)
  • GH130
  • Enzyme
  • Phosphorolysis
  • Mannan
  • Thermophilic
Abstract
Proteins in the glycoside hydrolase family 130 (GH130, CAZy database) have been proposed to perform the phosphorolysis of β-1,2 and β-1,4-mannosyl linkages between the mannose at the non-reducing end of substrates and mannose, glucose or N-acetylglucosamine residues, with the subsequent release of α-mannose-1-phosphate. In this study, we compare five different GH130 enzymes (CpMan130 A-E) encoded within the genome of Caldanaerobius polysaccharolyticus, a thermophilic anaerobic bacterium able to ferment mannan as the sole carbon source. Analysis of substrate specificity and end product release allowed for the identification of pathways involving GH130 enzymes in the metabolism of mannans with different structures by this organism. Mechanistic studies involving the binding order and amino acid mapping on a three-dimensional model of Man130B helped to elucidate the ordered sequential bi-bi mechanism utilized by these enzymes. Phylogenetic analysis of over 950 sequences assigned to the GH130 family, combined with differences in amino acid conservation and substrate specificity, revealed a new subgroup for this family, GH130_3, consisting of thermostable enzymes that act on β-1,2-linked manno-oligosaccharides. The genomic context of all genes in the proposed subgroup GH130_3 suggests that they appear in pairs, preceded by an ABC-like transporter.
Graduation Semester
2016-12
Type of Resource
text
Permalink
http://hdl.handle.net/2142/95627
Copyright and License Information
Copyright 2016 Hans Muller Paul

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Animal Sciences